Journal of Acute Disease
Antibiotic resistance pattern and biofilm formation among clinical Acinetobacter baumannii isolates: A cross-sectional study
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Abstract:
Objective: To investigate the pattern of antibiotic resistance and biofilm production capabilities of clinical Acinetobacter baumannii (A. baumannii) isolates in this study. Methods: A. baumannii isolates were collected from Tehran Imam Khomeini Hospital in this cross-sectional study, and the minimum inhibitory concentrations for 16 antibiotics were determined using Vitek2® systems. All isolates were analyzed for biofilm production, then presence of biofilm-associated genes, and classⅠand Ⅱ integron genes.
Results: 60 non-replicate A. baumannii isolates were included in this study. The resistance rates reached 100% for aztreonam, cefepime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, piperacillin, ticarcillin, and trimethoprim-sulfamethoxazole. A. baumannii isolates were most sensitive to colistin and rifampicin being the most effective treatments. Multi-drug resistant and extensively drug-resistant isolates accounted for 83.3% and 16.7%, respectively. Of the isolates, 91.6% formed biofilms, categorized as 10% strong, 31.6% moderate, and 50% weak. No correlation was found between antibiotic resistance and biofilm formation. The genes csuE, abaI, and ompA were prevalent, but their distribution was similar across biofilm categories. A relationship between Int1 and biofilm production was noted.
Conclusions: The high rates of antibiotic resistance and biofilmformation, alongside the presence of integrons including class ⅠandⅡ, underscore the necessity for ongoing monitoring of A.baumannii. Notably, class Ⅰintegron presence was significantly linked to biofilm formation. Further research is needed to explore the connection between antibiotic resistance and biofilm production in A. baumannii.
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