目的:基于转录组测序探究光动力疗法（photodynamic therapy，PDT）对人牙周炎性组织基因表达的影响，分析关键差异表达基因（differentially expressed genes，DEGs），并探寻PDT的潜在机制。 方法:临床收集健康牙周组织、牙周炎性组织和PDT治疗后牙周炎性组织各5例，进行转录组测序，并利用生物信息学分析进行枢纽基因筛选及富集分析。每组再收集15例组织，利用RT‑qPCR法验证测序结果。 结果:健康牙周组织和牙周炎性组织之间共检测出6 526个DEGs，牙周炎性组织和PDT治疗后牙周炎性组织之间共检测出1 209个DEGs，筛选出72个免疫相关差异基因，GO富集分析包括白细胞迁移、对细菌的防御反应等生物学过程；KEGG通路分析主要涉及细胞因子‑细胞因子受体相互作用、JAK‑STAT信号通路、IL‑17信号通路等通路。构建PPI网络并筛选出6个枢纽基因（S100A7、LCN2、LTF、DEFB4A、S100A12、S100A8），RT‑qPCR验证结果与测序结果一致。 结论:本研究确定了PDT治疗后人牙周炎性组织mRNA的表达谱，鉴定相关枢纽基因及功能分析，为探讨PDT调控牙周炎性组织的潜在机制提供参考。

Objective:To explore the effect of photodynamic therapy （PDT） on gene expression in human periodontitis tissues based on RNA sequencing， analyze key differentially expressed genes （DEGs）， and explore the potential mechanism of PDT. Methods:A total of 5 cases of healthy periodontal tissue，5 cases of periodontic tissue， and 5 cases of periodontic tissue treated with PDT were collected clinically， and RNA sequencing was performed. Bioinformatics analysis was used for hub gene screening and enrichment analysis.Another 15 cases of tissues for each group were collected and validated the sequencing results using RT‑qPCR. Results:A total of 6 526 DEGs were detected between healthy periodontal tissue and periodontic tissue， and 1 209 DEGs were detected between periodontic tissue and periodontic tissue after PDT treatment，and 72 immune related differentially expressed genes were screened. GO enrichment analysis included biological processes such as leukocyte migration and bacterial defense response； KEGG pathway analysis mainly involved cytokine-cytokine receptor interactions， JAK‑STAT signaling pathway， IL‑17 signaling pathway， and other pathways. A PPI network was constructed and screen out 6 hub genes （S100A7， LCN2， LTF， DEFB4A， S100A12， S100A8）. The RT‑qPCR validation results were consistent with the sequencing results. Conclusion:The study determines the expression profile of mRNA in periodontitis tissue after PDT treatment， and identifies of relevant hub genes and functional analysis， can provide new ideas for studying the potential mechanism of PDT regulating of periodontitis tissue.

R781.42

新疆第二医学院科研项目（ZR202406）；乌鲁木齐市中医药科技创新项目（ZYYMS?10）；乌鲁木齐市卫生健康委科技计划项目（202322）