目的:采用氩离子束凝固术（argon plasma coagulation，APC）烧灼气管模拟创伤性气管狭窄（traumatic tracheal stenosis，TTS），并观察气管在支气管镜下和大体解剖学的变化。此外，探讨了3D打印硅酮支架植入对TTS的治疗作用，以期为临床治疗TTS提供1种新的治疗策略。 方法:9只比格犬随机分为3组，每组3只。对照组：比格犬全麻后，通过支气管镜观察气管腔；模型组：比格犬全麻后使用APC烧灼进行造模；3D打印支架治疗组：比格犬全麻后使用APC烧灼进行造模，在第21天植入3D打印硅酮支架。在第1、7、14、21、28天用支气管镜观察气管腔。第28天通过苏木精‑伊红染色法和马松染色评估病理特征。 结果:APC烧灼气管的方法可以建立比格犬TTS模型。在第28天，模型组的狭窄程度约为92%~99%，3D打印支架治疗组中狭窄程度约为28%~36%。3组之间的组织病理学特征不同。 结论:APC烧灼气管建立比格犬TTS模型相对简单、可靠、成本低。3D打印支架可以明显缓解TTS的狭窄程度。

Objective:To investigate the effects of Argon Plasma Coagulation （APC） on simulated tracheal traumatic stenosis（TTS）， we observed the resulting tracheal changes through bronchoscopy and gross anatomical assessment， and further explored the use of 3D printed silicone stent implantation as a novel clinical treatment strategy for TTS. Methods:Nine Beagles were randomly divided into three groups， with three Beagles in each group. The control group underwent bronchoscopy to observe the tracheal lumen following general anesthesia. In the model group， modeling was performed using APC cauterization after general anesthesia. The 3D‑printed stent treatment group also underwent modeling with APC cauterization after general anesthesia， and 3D‑printed silicone stents were implanted on the 21st day. The tracheal lumen was observed via bronchoscopy on days 1，7，14，21， and 28. Pathological features were assessed using the hematoxylin‑eosin staining method and Masson staining on day 28. Results:APC cauterization of the trachea allowed for modeling of TTS in Beagles. At day 28， the degree of stenosis was approximately 92%‑99% in the model group and 28%‑36% in the 3D‑printed stent‑treated group. Histopathologic features were different between the three groups. Conclusions:APC cauterization of the trachea to establish a model of TTS in beagle dogs is relatively simple， reliable， and inexpensive. 3D printed stents can significantly alleviate the degree of stenosis in traumatic airway stenosis.

R318.08;R562.12

海南省科技厅重点研发项目（ZDYF2022SHFZ281）