Journal of Hainan Medical University
Issue 21,2023 Table of Contents
LI Yue
,
KANG Bi-qian
,
HE Xiao-xuan
,
HU Rui
,
XIAO Zhen
,
LUO Chen-liang
,
WU Gui-you
,
HUANG Zhong-shi
2023, 29(21):1-6.
DOI: 10.13210/j.cnki.jhmu.20231018.001
Abstract:
Objective： To investigate the mechanism of action of tetrahydroxy stilbene glycosides （TSG）in ameliorating neuronal damage in Alzheimer's disease rats by regulating MKK7 and JNK kinases. Methods： A total of 24‑month‑old 42 SD rats were randomly selected for the experiment in 7 groups： normal group， sham‑operated group， model group， positive drug group， low， medium and high dose TSG group at 0.033 g/kg，0.1 g/kg，0.3 g/kg. The model group and the TSG groups were established by stereotaxic Aβ25‑35 solution. After 28 days， the model rats were selected by passive avoidance test. After screening， each dosage group of TSG and positive drug group was given intragastrically according to the corresponding dosage， and the experiment was carried out after 28 days. The pathological changes of hippocampal CA1 region were observed by tissue staining， and the amount of MKK7 and JNK proteins and the expression content of MKK7 and JNK mRNA by histochemical method of protein， and qRT‑PCR assay.Results： （1） HE staining observation： Compared with the normal group and the sham‑operated group， the number of nerve cells in the model group decreased and arranged irregularly， the cell membrane shrank， and the nucleus deformed and dissolved.The number of neurons in the positive drug group and TSG group also increased significantly， the order is also relatively well. （2） From the results of the tunel staining experiments：The positive apoptotic cells in the model group were higher than control group and sham‑operated group， positive drug group and TSG drugs group was significantly smaller than that in the model group （P < 0.05）. （3） Compared with the control group and the virtual operation group， the MKK7 and JNK protein concentrations in the brain of the model group were increased（P<0.05） by data analysis of immunohistochemistry： Compared with the model group， the protein expression of positive drug and TSG each dose group were reduced （P<0.05）. （4）The results of QRT‑PCR data showed that the levels of MKK7 and JNK mRNA in the brain tissue of the model group were increased compared with the normal group and sham‑operated groups（P<0.05）.Conclusion： Stilbene glycoside has a certain effect on neuronal injury and repair which may be related to the changes of mRNA transcription and protein expression of MKK7 and JNK kinases.
Objective： To investigate the mechanism of action of tetrahydroxy stilbene glycosides （TSG）in ameliorating neuronal damage in Alzheimer's disease rats by regulating MKK7 and JNK kinases. Methods： A total of 24‑month‑old 42 SD rats were randomly selected for the experiment in 7 groups： normal group， sham‑operated group， model group， positive drug group， low， medium and high dose TSG group at 0.033 g/kg，0.1 g/kg，0.3 g/kg. The model group and the TSG groups were established by stereotaxic Aβ25‑35 solution. After 28 days， the model rats were selected by passive avoidance test. After screening， each dosage group of TSG and positive drug group was given intragastrically according to the corresponding dosage， and the experiment was carried out after 28 days. The pathological changes of hippocampal CA1 region were observed by tissue staining， and the amount of MKK7 and JNK proteins and the expression content of MKK7 and JNK mRNA by histochemical method of protein， and qRT‑PCR assay.Results： （1） HE staining observation： Compared with the normal group and the sham‑operated group， the number of nerve cells in the model group decreased and arranged irregularly， the cell membrane shrank， and the nucleus deformed and dissolved.The number of neurons in the positive drug group and TSG group also increased significantly， the order is also relatively well. （2） From the results of the tunel staining experiments：The positive apoptotic cells in the model group were higher than control group and sham‑operated group， positive drug group and TSG drugs group was significantly smaller than that in the model group （P < 0.05）. （3） Compared with the control group and the virtual operation group， the MKK7 and JNK protein concentrations in the brain of the model group were increased（P<0.05） by data analysis of immunohistochemistry： Compared with the model group， the protein expression of positive drug and TSG each dose group were reduced （P<0.05）. （4）The results of QRT‑PCR data showed that the levels of MKK7 and JNK mRNA in the brain tissue of the model group were increased compared with the normal group and sham‑operated groups（P<0.05）.Conclusion： Stilbene glycoside has a certain effect on neuronal injury and repair which may be related to the changes of mRNA transcription and protein expression of MKK7 and JNK kinases.
2023, 29(21):7-13.
DOI: 10.13210/j.cnki.jhmu.20230726.001
Abstract:
Objective： To explore the impacts of bone marrow mesenchymal stem cells （BMSCs） intervention on the neuronal withering and autophagy in rats after subarachnoid hemorrhage （SAH） in rats and its process.Methods： Forty‑eight male SD rats， were randomly assigned to the followings groups： Sham surgery （Sham） group， modeling （SAH） group， modeling group + bone marrow mesenchymal stem cells （BMSCs） group， the modeling group + BMSCs + AMPK inhibitor （Compound C） group， with twelve rats in each group. Except Sham group， other groups formed SAH samples with intravascular points. Compound C and 10 μL of normal saline were injected into the left lateral ventricle of rats using a stereoscope brain machine 30 minutes before modeling in compound C and BMSCs groups，respectively. And 2 μL of cell suspension at a concentration of 1×108/mL was injected into the ventricles of the brain 1 hour after the model was established. The severity of brain swelling in rats was observed. Garcia were used to test the neural function of rats. TUNEL staining was used to monitor neuronal apoptosis in the rat hippocampal gyrus. Immunohistochemical fluorescence reflected the expression of two proteins， LC3‑II and Beclin‑1， in rat hippocampal gyrus. Western blotting was applied to measure the expressions of autophagy‑associated proteins in the AMPK pathway. Results： Compared with the group undergoing sham‑surgery， brain edema worsened in the model group； neuronal apoptosis rate was increased； neural function was weakened. Protein granules decreased （P<0.05） and expression levels of p‑AMPK and other proteins decreased （P<0.05）； brain swelling and neuronal apoptosis were reduced in the BMSCs group by comparison with the modeling group’s， with substantial elevation in the expression of proteins comprising LC3‑Ⅱ， Beclin‑1， and p‑AMPK. And the standard of p‑mTOR protein expression was considerably lessened （P<0.05）； rats belonging to group compound C showed increased brain swelling which is relative to that of BMSCs group， increased neuronal apoptosis， decreased neuronal function （P<0.05）， increased p‑AMPK protein expression， and decreased LC3‑Ⅱ， Beclin‑1， and p‑mTOR protein expression （P<0.05）. Conclusion： BMSCs transplantation can reduce neuronal apoptosis after SAH； its principle may be to activate AMPK/mTOR pathway， strengthen autophagy of neurons， and thus inhibit their transformation to apoptosis.
Objective： To explore the impacts of bone marrow mesenchymal stem cells （BMSCs） intervention on the neuronal withering and autophagy in rats after subarachnoid hemorrhage （SAH） in rats and its process.Methods： Forty‑eight male SD rats， were randomly assigned to the followings groups： Sham surgery （Sham） group， modeling （SAH） group， modeling group + bone marrow mesenchymal stem cells （BMSCs） group， the modeling group + BMSCs + AMPK inhibitor （Compound C） group， with twelve rats in each group. Except Sham group， other groups formed SAH samples with intravascular points. Compound C and 10 μL of normal saline were injected into the left lateral ventricle of rats using a stereoscope brain machine 30 minutes before modeling in compound C and BMSCs groups，respectively. And 2 μL of cell suspension at a concentration of 1×108/mL was injected into the ventricles of the brain 1 hour after the model was established. The severity of brain swelling in rats was observed. Garcia were used to test the neural function of rats. TUNEL staining was used to monitor neuronal apoptosis in the rat hippocampal gyrus. Immunohistochemical fluorescence reflected the expression of two proteins， LC3‑II and Beclin‑1， in rat hippocampal gyrus. Western blotting was applied to measure the expressions of autophagy‑associated proteins in the AMPK pathway. Results： Compared with the group undergoing sham‑surgery， brain edema worsened in the model group； neuronal apoptosis rate was increased； neural function was weakened. Protein granules decreased （P<0.05） and expression levels of p‑AMPK and other proteins decreased （P<0.05）； brain swelling and neuronal apoptosis were reduced in the BMSCs group by comparison with the modeling group’s， with substantial elevation in the expression of proteins comprising LC3‑Ⅱ， Beclin‑1， and p‑AMPK. And the standard of p‑mTOR protein expression was considerably lessened （P<0.05）； rats belonging to group compound C showed increased brain swelling which is relative to that of BMSCs group， increased neuronal apoptosis， decreased neuronal function （P<0.05）， increased p‑AMPK protein expression， and decreased LC3‑Ⅱ， Beclin‑1， and p‑mTOR protein expression （P<0.05）. Conclusion： BMSCs transplantation can reduce neuronal apoptosis after SAH； its principle may be to activate AMPK/mTOR pathway， strengthen autophagy of neurons， and thus inhibit their transformation to apoptosis.
SUN Dong-qi
,
ZHOU Xiao-ling
,
WU Teng
,
LI Ling-chang
,
LI Ze-peng
,
SUN Su-hong
,
WANG Yue-ming
,
LIU Lin
,
MO Yi-ling
,
YU Jing-fang
2023, 29(21):14-14.
DOI: 10.13210/j.cnki.jhmu.20231009.001
Abstract:
Objective： To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non‑alcoholic fatty liver disease （NAFLD）. Methods：High-fat feed was used to induce NAFLD in rats， Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index. Liver pathology was observed， while serum transaminase and blood lipid levels were measured. The protein expression levels of PINK1， Parkin， and LC‑3Ⅱ in rat liver were detected using Western Blot analysis. Results： Compared with the control group， the NAFLD rats exhibited a significant increase in body weight， liver wet weight， liver index， transaminase levels， and blood lipid levels. The expression levels of PINK1， Parkin， and LC3‑II protein were significantly decreased （P<0.01）.Following intervention with Lizhong Tang， rats in each herbal treatment group displayed a decrease in body weight， liver wet weight， liver index， serum transaminase， and blood lipid levels.The expression levels of PINK1， Parkin， and LC‑3Ⅱ rebounded （P<0.05）， with the high‑dose group demonstrating the most pronounced effects（P<0.01）. Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure， swollen hepatocytes， disordered arrangement， and a multitude of varying‑sized lipid vacuoles within the cytoplasm. Conversely， rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology， with the high‑dose group showing the most notable enhancement. Conclusion： Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.
Objective： To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non‑alcoholic fatty liver disease （NAFLD）. Methods：High-fat feed was used to induce NAFLD in rats， Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index. Liver pathology was observed， while serum transaminase and blood lipid levels were measured. The protein expression levels of PINK1， Parkin， and LC‑3Ⅱ in rat liver were detected using Western Blot analysis. Results： Compared with the control group， the NAFLD rats exhibited a significant increase in body weight， liver wet weight， liver index， transaminase levels， and blood lipid levels. The expression levels of PINK1， Parkin， and LC3‑II protein were significantly decreased （P<0.01）.Following intervention with Lizhong Tang， rats in each herbal treatment group displayed a decrease in body weight， liver wet weight， liver index， serum transaminase， and blood lipid levels.The expression levels of PINK1， Parkin， and LC‑3Ⅱ rebounded （P<0.05）， with the high‑dose group demonstrating the most pronounced effects（P<0.01）. Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure， swollen hepatocytes， disordered arrangement， and a multitude of varying‑sized lipid vacuoles within the cytoplasm. Conversely， rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology， with the high‑dose group showing the most notable enhancement. Conclusion： Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.
2023, 29(21):15-15.
DOI: 10.13210/j.cnki.jhmu.20230911.001
Abstract:
Objective： To explore the effect of Qingre Quzhuo Capsule （QRQZ） in the treatment of non‑alcoholic steatohepatitis （NASH） and to explore its mechanism from the perspective of inhibiting ferroptosis. Methods： 60 C57BL/6J mice were randomly divided into 6 groups： Control， model， ferroptosis inhibitor （Fer‑1， 10 mg/kg， gavage）， low‑dose QRQZ （QRQZL， 482 mg/kg， gavage）， medium‑dose QRQZ （QRQZM， 964 mg/kg， gavage）， and high‑dose QRQZ （QRQZH， 1 929 mg/kg， gavage）. The control group was given normal diet， and the other experimental groups were given MCD diet. The whole administration process lasted for 6 weeks. The body weight of mice was counted every week. After 6 weeks， the blood of mice was collected， and the serum was separated to determine ALT and AST. The liver of mice was weighed and fixed. The same part was stained with HE and Oil Red O to observe the pathological changes of liver tissue. The levels of TC， TG， total iron， GSH and GSSG in liver tissue were measured by kit. The expression of Gpx4 mRNA was detected by RT‑qPCR， and the expression of FTH1， FTL and GPX4 protein was detected by Western blotting. Results： Compared with the model group， after treatment with Fer‑1 and QRQZ， the body weight and liver index of NASH mice increased， the liver tissue lesions were alleviated， TC， TG， ALT and AST were improved， the liver tissue iron level decreased significantly， the relative levels of FTH1 and FTL proteins increased significantly， GSH/GSSG increased significantly， and the expression of Gpx4 mRNA and GPX4 protein increased significantly.Conclusion： QRQZ alleviates liver injury in NASH mice by promoting the expression of GSH/GPX4 antioxidant system and inhibiting ferroptosis.
Objective： To explore the effect of Qingre Quzhuo Capsule （QRQZ） in the treatment of non‑alcoholic steatohepatitis （NASH） and to explore its mechanism from the perspective of inhibiting ferroptosis. Methods： 60 C57BL/6J mice were randomly divided into 6 groups： Control， model， ferroptosis inhibitor （Fer‑1， 10 mg/kg， gavage）， low‑dose QRQZ （QRQZL， 482 mg/kg， gavage）， medium‑dose QRQZ （QRQZM， 964 mg/kg， gavage）， and high‑dose QRQZ （QRQZH， 1 929 mg/kg， gavage）. The control group was given normal diet， and the other experimental groups were given MCD diet. The whole administration process lasted for 6 weeks. The body weight of mice was counted every week. After 6 weeks， the blood of mice was collected， and the serum was separated to determine ALT and AST. The liver of mice was weighed and fixed. The same part was stained with HE and Oil Red O to observe the pathological changes of liver tissue. The levels of TC， TG， total iron， GSH and GSSG in liver tissue were measured by kit. The expression of Gpx4 mRNA was detected by RT‑qPCR， and the expression of FTH1， FTL and GPX4 protein was detected by Western blotting. Results： Compared with the model group， after treatment with Fer‑1 and QRQZ， the body weight and liver index of NASH mice increased， the liver tissue lesions were alleviated， TC， TG， ALT and AST were improved， the liver tissue iron level decreased significantly， the relative levels of FTH1 and FTL proteins increased significantly， GSH/GSSG increased significantly， and the expression of Gpx4 mRNA and GPX4 protein increased significantly.Conclusion： QRQZ alleviates liver injury in NASH mice by promoting the expression of GSH/GPX4 antioxidant system and inhibiting ferroptosis.
2023, 29(21):16-21.
DOI: 10.13210/j.cnki.jhmu.20230901.001
Abstract:
Objective：To prepare resveratrol polymer micelles with tissue adhesion and determine the content of resveratrol in the micelles by HPLC. Methods： The micelle adhesion experiment was carried out by polylysine orifice plate experiment and small animal fluorescence imaging method， and the micelle prescription was optimized to obtain resveratrol micelles with good adhesion. The separation was performed on a Shiseido SPOLAR C18 column （150 mm × 4.6 mm， 5 μm） with methanol‑water （42∶58） as the mobile phase. The flow rate was 1.0 mL/min， the detection wavelength was 305 nm， the column temperature was 35 ℃， and the injection volume was 10 μL. Results： Resveratrol micelles prepared with F127 alone had the best adhesion. The peak area and concentration of resveratrol had a good linear relationship in the concentration range of 10~200 μg/mL （r=0.9996）. The specificity， precision， recovery and stability all met the methodological requirements. Conclusion： In this experiment， resveratrol micelles with tissue adhesion were successfully prepared， and a method for the determination of resveratrol content in micelles was established. The method is accurate， rapid and simple.
Objective：To prepare resveratrol polymer micelles with tissue adhesion and determine the content of resveratrol in the micelles by HPLC. Methods： The micelle adhesion experiment was carried out by polylysine orifice plate experiment and small animal fluorescence imaging method， and the micelle prescription was optimized to obtain resveratrol micelles with good adhesion. The separation was performed on a Shiseido SPOLAR C18 column （150 mm × 4.6 mm， 5 μm） with methanol‑water （42∶58） as the mobile phase. The flow rate was 1.0 mL/min， the detection wavelength was 305 nm， the column temperature was 35 ℃， and the injection volume was 10 μL. Results： Resveratrol micelles prepared with F127 alone had the best adhesion. The peak area and concentration of resveratrol had a good linear relationship in the concentration range of 10~200 μg/mL （r=0.9996）. The specificity， precision， recovery and stability all met the methodological requirements. Conclusion： In this experiment， resveratrol micelles with tissue adhesion were successfully prepared， and a method for the determination of resveratrol content in micelles was established. The method is accurate， rapid and simple.
2023, 29(21):22-30.
DOI: 10.13210/j.cnki.jhmu.20230807.001
Abstract:
Objective： To investigate the mechanism of action and material basis of AiTongXiao granule in the treatment of hepatocellular carcinoma （HCC） based on network pharmacology and transplanted liver cancer rat model. Methods： TCMSP database was used to screen out effective components and its corresponding potential pharmaceutical targets， and databases including Gene Cards， OMIN， Drugbank and TTD were further used to collect HCC‑related drug targets. The intersecting targets were obtained by mapping the drug and disease targets. The component‑targets network was constructed and visualized by Cytoscape 3.8.2 software. Protein‑protein interaction （PPI） network was built by STRING online platform， and the topological relationship and core targets were analyzed and screened by using CytoNCA software. In addition， Metascape database was used to perform gene ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis of the core targets. At last， A transplanted liver cancer rat model was established by using Walker‑256 cell line and treated by AiTongXiao granule for 15 days. Western blot was used to further compare the expression levels of AKT， pAKT， p53， p‑p53， ERK1/2 and ERK1/2 in the tumor between the treatment group and the control group.Results：A total of 257 active components were obtained from AiTongXiao granule， corresponding to 294 drug targets. Meanwhile， 231 of the 7 993 HCC disease targets were screened out between AiTongXiao granule drug and HCC disease targets. Eleven core targets including AKT1， IL6， TP53， MAPK3， TNF， JUN ， CASP3，MAPK1，MYC，PTGS2 and MMP9 were further obtained by median screening. GO and KEGG analysis results showed that these core targets enriched to HBV， TNF and cancer related pathways. The rat transplanted liver cancer model results indicated significant downregulation for AKT， p‑AKT， pERK1/2， and significant upregulation of p‑p53 after AiTongXiao granule treatment （P<0.05）.Conclusion： AiTongXiao granule could act to multiple cancer related pathways， and AKT， p53 and ERK1/2 were validated to be regulated by ATXF in rat model. The mechanism may be through the regulation of the above signaling pathways to exert anti‑liver cancer effect.
Objective： To investigate the mechanism of action and material basis of AiTongXiao granule in the treatment of hepatocellular carcinoma （HCC） based on network pharmacology and transplanted liver cancer rat model. Methods： TCMSP database was used to screen out effective components and its corresponding potential pharmaceutical targets， and databases including Gene Cards， OMIN， Drugbank and TTD were further used to collect HCC‑related drug targets. The intersecting targets were obtained by mapping the drug and disease targets. The component‑targets network was constructed and visualized by Cytoscape 3.8.2 software. Protein‑protein interaction （PPI） network was built by STRING online platform， and the topological relationship and core targets were analyzed and screened by using CytoNCA software. In addition， Metascape database was used to perform gene ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis of the core targets. At last， A transplanted liver cancer rat model was established by using Walker‑256 cell line and treated by AiTongXiao granule for 15 days. Western blot was used to further compare the expression levels of AKT， pAKT， p53， p‑p53， ERK1/2 and ERK1/2 in the tumor between the treatment group and the control group.Results：A total of 257 active components were obtained from AiTongXiao granule， corresponding to 294 drug targets. Meanwhile， 231 of the 7 993 HCC disease targets were screened out between AiTongXiao granule drug and HCC disease targets. Eleven core targets including AKT1， IL6， TP53， MAPK3， TNF， JUN ， CASP3，MAPK1，MYC，PTGS2 and MMP9 were further obtained by median screening. GO and KEGG analysis results showed that these core targets enriched to HBV， TNF and cancer related pathways. The rat transplanted liver cancer model results indicated significant downregulation for AKT， p‑AKT， pERK1/2， and significant upregulation of p‑p53 after AiTongXiao granule treatment （P<0.05）.Conclusion： AiTongXiao granule could act to multiple cancer related pathways， and AKT， p53 and ERK1/2 were validated to be regulated by ATXF in rat model. The mechanism may be through the regulation of the above signaling pathways to exert anti‑liver cancer effect.
2023, 29(21):31-43.
DOI: 10.13210/j.cnki.jhmu.20230721.003
Abstract:
Objective： Constructing a prognostic model for gastric cancer （GC） based on cuproptosis‑related LncRNAs （CRLs）and predict the traditional Chinese medicine that regulates cuproptosis‑related genes （CRGs）. Methods： Clinical data and RNA‑seq of 443 GC cases were obtained from The Cancer Genome Atlas database， and CRLs were screened by Pearson analysis， Cox regression， and least absolute shrinkage and selection operator regression to construct a risk model to predict GC prognosis， and the nomogram was constructed by combining risk scores and clinical characteristics. The accuracy of the model was validated by the receiver operating characteristic curve， Kaplan‑Meier curves and C‑index. The correlation of risk scores with immune infiltration， immune checkpoint gene expression and chemotherapy/targeted agents was assesssed. The Coremine Medical database was applied to predict potential traditional Chinese medicine that regulates CRGs. Results： Risk models for GC were constructed based on the risk scores of seven CRLs （AP001107.9， VCAN‑AS1， AC016394.2， LINC02675， AC100814.1， HAGLR， and LINC01094）. The AUC of the risk model predicting 1‑， 3‑， and 5‑year survival in GC patients was 0.720， 0.682， and 0.711，respectively and its prognostic value was better than age， grade classification， and TNM stage. The AUC of the risk model combining age and TNM stage to predict 1‑year survival in GC patients was 0.793. The risk score correlated with the degree of enrichment of immune cells such as tumor‑infiltrating lymphocytes and regulatory T cells and the expression of 22 immune checkpoint genes such as LAG3， ICOS， CD28， NRP1 and the sensitivity of 13 chemotherapeutic/targeted agents. There were 58 traditional Chinese medicine with potential regulatory effects on CRGs， mainly for clearing heat and detoxing， promoting blood circulation and relieving pain， which were mainly attributed to the liver， spleen and lung meridians. Spirulina and osthole had potential regulatory effects on FDX1， a key gene in the death mechanism of cuproptosis.Conclusion： A risk signature constructed based on seven CRLs could assess the prognosis and immunity of GC， and Spirulina and Serpentine may have important regulatory efficacy on the mechanism of copper cuproptosis.
Objective： Constructing a prognostic model for gastric cancer （GC） based on cuproptosis‑related LncRNAs （CRLs）and predict the traditional Chinese medicine that regulates cuproptosis‑related genes （CRGs）. Methods： Clinical data and RNA‑seq of 443 GC cases were obtained from The Cancer Genome Atlas database， and CRLs were screened by Pearson analysis， Cox regression， and least absolute shrinkage and selection operator regression to construct a risk model to predict GC prognosis， and the nomogram was constructed by combining risk scores and clinical characteristics. The accuracy of the model was validated by the receiver operating characteristic curve， Kaplan‑Meier curves and C‑index. The correlation of risk scores with immune infiltration， immune checkpoint gene expression and chemotherapy/targeted agents was assesssed. The Coremine Medical database was applied to predict potential traditional Chinese medicine that regulates CRGs. Results： Risk models for GC were constructed based on the risk scores of seven CRLs （AP001107.9， VCAN‑AS1， AC016394.2， LINC02675， AC100814.1， HAGLR， and LINC01094）. The AUC of the risk model predicting 1‑， 3‑， and 5‑year survival in GC patients was 0.720， 0.682， and 0.711，respectively and its prognostic value was better than age， grade classification， and TNM stage. The AUC of the risk model combining age and TNM stage to predict 1‑year survival in GC patients was 0.793. The risk score correlated with the degree of enrichment of immune cells such as tumor‑infiltrating lymphocytes and regulatory T cells and the expression of 22 immune checkpoint genes such as LAG3， ICOS， CD28， NRP1 and the sensitivity of 13 chemotherapeutic/targeted agents. There were 58 traditional Chinese medicine with potential regulatory effects on CRGs， mainly for clearing heat and detoxing， promoting blood circulation and relieving pain， which were mainly attributed to the liver， spleen and lung meridians. Spirulina and osthole had potential regulatory effects on FDX1， a key gene in the death mechanism of cuproptosis.Conclusion： A risk signature constructed based on seven CRLs could assess the prognosis and immunity of GC， and Spirulina and Serpentine may have important regulatory efficacy on the mechanism of copper cuproptosis.
8 Meta‑analysis of the acupoint application therapy for stable chronic obstructive pulmonary disease
2023, 29(21):44-52.
DOI: 10.13210/j.cnki.jhmu.20230515.001
Abstract:
Objective： To systematically evaluate the clinical efficacy and safety of acupoint application in the treatment of stable chronic obstructive pulmonary disease.Methods： A comprehensive search of various international and domestic databases was conducted， the search time is limited to recent ten years， collecting traditional Chinese medicine acupoint application in the treatment of chronic obstructive pulmonary disease in stable phase of randomized controlled trials.Literature screening， information extraction and literature quality evaluation were carried out independently by two researchers， and meta-analysis was carried out by Revman software.Results： A total of 42 RCTs with a total of 4 192 patients were included. The results of meta-analysis showed that， compared with the routine treatment of western medicine， the addition of acupoint application therapy can significantly increase the effective rate［RR=1.23，95%CI（1.19，1.27），P<0.000 01］ and FEV1/FVC［MD=4.46，95%CI（3.17，5.76），P<0.000 01］，and significantly improved BODE index［MD=-0.63，95%CI（-0.87，-0.40），P<0.000 01］，SGRQ score［MD=-6.77，95%CI（-9.81，-3.72），P<0.000 01］，CAT score［MD=-3.33，95%CI（-3.87，-2.79），P<0.000 01］ and TCM syndrome integral［MD=-3.51，95%CI（-3.96，-3.06），P<0.000 01］.The differences is statistically significant and the safety is good.Conclusion： On the basis of routine treatment of COPD western medicine， acupoint application therapy can significantly improve the clinical symptoms of patients with less adverse reactions. However， due to the limitations of the research， high‑quality research is still needed to provide further evidence.
Objective： To systematically evaluate the clinical efficacy and safety of acupoint application in the treatment of stable chronic obstructive pulmonary disease.Methods： A comprehensive search of various international and domestic databases was conducted， the search time is limited to recent ten years， collecting traditional Chinese medicine acupoint application in the treatment of chronic obstructive pulmonary disease in stable phase of randomized controlled trials.Literature screening， information extraction and literature quality evaluation were carried out independently by two researchers， and meta-analysis was carried out by Revman software.Results： A total of 42 RCTs with a total of 4 192 patients were included. The results of meta-analysis showed that， compared with the routine treatment of western medicine， the addition of acupoint application therapy can significantly increase the effective rate［RR=1.23，95%CI（1.19，1.27），P<0.000 01］ and FEV1/FVC［MD=4.46，95%CI（3.17，5.76），P<0.000 01］，and significantly improved BODE index［MD=-0.63，95%CI（-0.87，-0.40），P<0.000 01］，SGRQ score［MD=-6.77，95%CI（-9.81，-3.72），P<0.000 01］，CAT score［MD=-3.33，95%CI（-3.87，-2.79），P<0.000 01］ and TCM syndrome integral［MD=-3.51，95%CI（-3.96，-3.06），P<0.000 01］.The differences is statistically significant and the safety is good.Conclusion： On the basis of routine treatment of COPD western medicine， acupoint application therapy can significantly improve the clinical symptoms of patients with less adverse reactions. However， due to the limitations of the research， high‑quality research is still needed to provide further evidence.
2023, 29(21):53-59.
DOI: 10.13210/j.cnki.jhmu.20221114.002
Abstract:
Parkinson's disease （PD） is a chronic progressive neurodegenerative disease. Deep brain stimulation （DBS） is an effective treatment for patients with advanced PD. There are many DBS targets for PD， including subthalamic nucleus， globus pallidus， meso⁃ventral thalamic nucleus， pontine peduncle nucleus， posterior subthalamic areas and zonation of undetermined zone. This paper summarizes the efficacy of each target in the treatment of PD with DBS， not only conducts a systematic analysis and comparison of motor symptoms， and also carries out a detailed description of the efficacy of non⁃motor symptoms， so as to provide a personalized treatment basis for PD patients to select appropriate target points in DBS.
Parkinson's disease （PD） is a chronic progressive neurodegenerative disease. Deep brain stimulation （DBS） is an effective treatment for patients with advanced PD. There are many DBS targets for PD， including subthalamic nucleus， globus pallidus， meso⁃ventral thalamic nucleus， pontine peduncle nucleus， posterior subthalamic areas and zonation of undetermined zone. This paper summarizes the efficacy of each target in the treatment of PD with DBS， not only conducts a systematic analysis and comparison of motor symptoms， and also carries out a detailed description of the efficacy of non⁃motor symptoms， so as to provide a personalized treatment basis for PD patients to select appropriate target points in DBS.
2023, 29(21):60-63.
DOI: 10.13210/j.cnki.jhmu.20221010.001
Abstract:
Subchorionic hematoma is a common cause of vaginal bleeding during pregnancy， with an incidence of 4% to 22%. This paper focuses on the ultrasonic characteristics of subchorionic hematoma as well as the correlation and possible mechanism of subchorionic hematoma and pregnancy complications such as abortion， premature delivery and placental abruption， so as to provide help for clinicians to better understand about subchorionic hematoma.
Subchorionic hematoma is a common cause of vaginal bleeding during pregnancy， with an incidence of 4% to 22%. This paper focuses on the ultrasonic characteristics of subchorionic hematoma as well as the correlation and possible mechanism of subchorionic hematoma and pregnancy complications such as abortion， premature delivery and placental abruption， so as to provide help for clinicians to better understand about subchorionic hematoma.
2023, 29(21):64-68.
DOI: 10.13210/j.cnki.jhmu.20221026.001
Abstract:
Knee osteoarthritis， as a chronic disabling disease， not only brings trouble to the patients， but also brings great psychological and economic burden to their families. Although there are various treatment methods， the curative effect is not good， mainly relieving symptoms， without effective intervention in intervene the disease progression. Necroptosis and ferroptosis are two new pathways of programmed cell death discovered in recent years，they play a key role in the occurrence and development of knee osteoarthritis and also provide a new opportunity for the prevention and treatment of knee osteoarthritis.This article reviews necroptosis and ferroptosis and their related studies in knee osteoarthritis.
Knee osteoarthritis， as a chronic disabling disease， not only brings trouble to the patients， but also brings great psychological and economic burden to their families. Although there are various treatment methods， the curative effect is not good， mainly relieving symptoms， without effective intervention in intervene the disease progression. Necroptosis and ferroptosis are two new pathways of programmed cell death discovered in recent years，they play a key role in the occurrence and development of knee osteoarthritis and also provide a new opportunity for the prevention and treatment of knee osteoarthritis.This article reviews necroptosis and ferroptosis and their related studies in knee osteoarthritis.
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