目的:探讨锌指蛋白转录因子4（kruppel‑like factor 4， KLF4）/血管内皮生长因子（vascular endothelial growth factor， VEGF）信号轴在血小板源性生长因子‑BB（platelet‑derived growth factor‑BB， PDGF‑BB）诱导的人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）迁移和血管新生中的作用。 方法:采用空白组及不同浓度的PDGF‑BB（5、10、20和40 ng/mL）刺激脐静脉内皮细胞建立细胞迁移和血管新生模型。通过小干扰RNA下调KLF4表达，分别采取CCK8实验检测细胞增殖，基质胶血管形成实验检测细胞成管能力，划痕实验评估细胞迁移情况。Western blot 检测PDGF‑BB刺激后内皮细胞中增殖细胞核抗原（proliferating cell nuclear antigen， PCNA）、KLF4和VEGF蛋白表达。 结果:与空白组比较，不同浓度的PDGF‑BB刺激能明显促进内皮细胞的增殖、迁移和管腔形成能力；明显上调PCNA、KLF4和VEGF蛋白表达，且呈剂量和时间依赖关系。运用si‑RNA沉默KLF4表达，可以明显抑制PDGF‑BB 诱导的内皮细胞迁移和血管新生，下调PDGF‑BB诱导的PCNA和VEGF的蛋白表达水平。 结论:PDGF‑BB通过激活KLF4/VEGF信号轴诱导HUVECs增殖、迁移和血管生成。

Objective:To investigate the effect of kruppel‑like factor 4（KLF4）/ vascular endothelial growth factor （VEGF） signaling axis on the migration and angiogenesis of human umbilical vein endothelial cells （HUVECs） induced by platelet‑derived growth factor‑BB （PDGF‑BB）. Methods:HUVECs were stimulated with the blank group and different concentrations of PDGF‑BB （5， 10， 20， 40 ng/mL） to establish cell migration and angiogenesis models. The expression of KLF4 was down‑regulated by small interfering RNA. The cell proliferation rate was detected by CCK8 cell counting kit and migration was assayed by scratch test. The tube formation ability was detected by lumen formation assay. The expressions of proliferation cell nuclear antigen （PCNA）， KLF4 and VEGF were evaluated by Western blot. Results:Compared to the blank group， PDGF‑BB significantly increased the cell proliferation and migration， promoted tube formation， and up‑regulated the protein expression of PCNA， KLF4 and VEGF， and it shows a dose and time-dependent relationship. Silencing KLF4 expression with a specific small interfering RNA （siRNA） inhibited PCNA and VEGF expression and， importantly， suppressed PDGF‑BB‑induced cell migration and angiogenesis. Conclusion:PDGF‑BB induces HUVECs proliferation， migration and angiogenesis via activating KLF4/VEGF signaling axis.

R329.2

中部战区总医院育英计划项目（ZZYC202102）；湖北省自然科学基金青年项目（2021CFB161）