目的:探讨烯醇化酶2（enolase2，ENO2）对口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）细胞增殖的影响。 方法:收集20例OSCC患者的肿瘤组织，同时也从河子大学第一附属医院获取了20例相对应的癌旁组织，qPCR检测2种组织中ENO2表达。在SCC15细胞中分别转染ENO2干扰序列（si⁃ENO2组）及阴性对照干扰序列（si⁃NC组），经转染效率评估后，构建敲低ENO2表达的口腔鳞状细胞癌细胞株SCC15，Western blot和qPCR验证ENO2干扰效率。细胞计数试剂盒⁃8（CCK⁃8）增殖实验、划痕愈合实验和Transwell 侵袭实验检测si⁃ENO2组和si⁃NC组细胞的生长增殖和迁移、侵袭能力。 结果:OSCC组织中ENO2相对表达量明显高于正常癌旁组织。转染ENO2干扰片段后，si⁃ENO2组细胞的生长增殖和迁移侵袭较si⁃NC组明显降低（P<0.05）。 结论:ENO2在OSCC组织中高表达，敲低ENO2表达能够抑制OSCC细胞的生长、增殖和迁移功能，且可能通过细胞周期阻滞而严重损害癌细胞增殖影响OSCC的发生及进展，ENO2可能是OSCC新的治疗靶点。

Objective:To investigate the impact of enolase 2 （ENO2） on the proliferation of oral squamous cell carcinoma cells （OSCC）. Methods:Tumor tissues from twenty patients with OSCC and the corresponding adjacent normal tissues were collected from First Affiliated Hospital of Shihezi University. The expression level of ENO2 in both tissue types was measured using qPCR. SCC15 cells were transfected with ENO2⁃specific interfering sequence （si⁃ENO2 group） or negative control interfering sequence （si⁃NC group）. After assessment of transfection efficiency， an oral squamous cell carcinoma SCC15 cell line was constructed with ENO2 knockdown， verification of ENO2 interference efficiency by Western blot and qPCR. Assessment of cell growth， proliferation， migration， and invasion in si⁃ENO2 and si⁃NC groups were detected by CCK⁃8 proliferation assay， scratch healing assay， and transwell invasion assay. Results:The expression level of ENO2 in OSCC was significantly higher compared to adjacent normal tissues. Following transfection with ENO2 interfering fragments， the growth， proliferation， migration， and invasion of cells in the si⁃ENO2 group were significantly reduced compared to the si⁃NC group （P<0.05）. Conclusion:ENO2 is overexpressed in OSCC. Silencing ENO2 expression can inhibit OSCC cell growth， proliferation， and migration. This may significantly impair cancer cell proliferation and influence OSCC development and progression by inducing cell cycle arrest. Therefore， ENO2 represents a potential therapeutic target for OSCC.

R739.8

国家自然科学基金（82160572）；兵团科技计划资助基金（2023ZD017）