目的:分析TRIM56在多种肿瘤中的作用，并验证其在食管癌组织和细胞中的表达及功能。 方法:通过UCSC、TCGA、GTEx、GEPIA2等数据库，从基因表达、预后分析、免疫细胞浸润等角度全面分析TRIM56在不同肿瘤中的作用。采用qRT‑PCR和Western blot检测Eca‑109细胞中TRIM56的表达，免疫组化检测食管癌组织及癌旁组织中TRIM56的水平。通过Transwell和划痕实验评估过表达和沉默TRIM56对Eca‑109细胞侵袭与迁移能力的影响。 结果:大多数肿瘤组织中TRIM56表达明显高于正常组织（P<0.05）。TRIM56高表达与部分肿瘤的不良预后、免疫细胞浸润明显相关，并参与先天免疫、肿瘤增殖、自噬等生物过程。免疫组化显示，TRIM56在食管鳞癌组织中明显高于癌旁组织。功能实验表明，过表达TRIM56增强了Eca‑109细胞的侵袭和迁移能力，而沉默TRIM56则明显抑制其侵袭和迁移能力。 结论:TRIM56的表达与癌症患者的临床预后和免疫细胞浸润密切相关。在食管癌中，TRIM56高表达促进细胞侵袭和迁移，可能作为癌基因发挥作用。TRIM56有望成为食管癌研究的潜在诊断和治疗靶点，有助于深入理解其在癌症发生、发展中的角色。

Objective:To investigate the role of TRIM56 across various tumors and validate its expression and function in esophageal cancer tissues and cells. Methods:The role of TRIM56 in different tumors was comprehensively analyzed through databases including UCSC， TCGA， GTEx， and GEPIA2， focusing on gene expression， prognostic significance， and immune cell infiltration. The expression of TRIM56 in Eca‑109 cells was assessed using qRT‑PCR and Western blot， while immunohistochemistry was employed to analyze TRIM56 levels in esophageal cancer tissues and adjacent non‑cancerous tissues. Transwell and scratch assays were conducted to evaluate the effects of TRIM56 overexpression and silencing on the invasive and migratory abilities of Eca‑109 cells. Results:TRIM56 expression was significantly elevated in most tumor tissues compared to normal tissues （P<0.05）. High TRIM56 expression correlated with poor prognosis and increased immune cell infiltration in several tumor types. TRIM56 was found to be involved in critical biological processes such as innate immunity， tumor proliferation， and autophagy. Immunohistochemistry demonstrated that TRIM56 levels were significantly higher in esophageal squamous carcinoma tissues compared to adjacent non‑cancerous tissues. Functional assays revealed that overexpression of TRIM56 enhanced the invasive and migratory abilities of Eca‑109 cells， while silencing TRIM56 markedly inhibited these capabilities. Conclusion:TRIM56 expression is closely associated with clinical prognosis and immune cell infiltration in cancer patients. In esophageal cancer， high TRIM56 expression promotes cell invasion and migration， suggesting that TRIM56 functions as an oncogene. TRIM56 holds promise as a potential diagnostic and therapeutic target in esophageal cancer， offering new insights into its role in tumor progression.

R735.1

2020广西壮族自治区中青年教师基础能力提升项目（2020KY13027）