目的:探讨S100钙结合蛋白A8（S100A8）对膀胱癌增殖、侵袭和迁移的影响及作用机制。 方法:通过TCGA数据库、HPA数据库及石河子大学第一附属医院泌尿外科的膀胱癌患者数据探究S100A8在膀胱癌中的表达情况。选用CCLE数据库筛选的S100A8表达较高的膀胱癌细胞系HT‑1376以及人正常尿路上皮细胞株SV‑HUC‑1，Western blot法与qRT‑PCR实验验证其表达情况。取HT‑1376细胞分为敲低（KD）组、阳性对照（NC）组、过表达（OE）组及正常组，对其进行敲低和过表达的慢病毒转染，Western blot与qRT‑PCR实验检测其转染效率。CCK‑8法与EdU增殖实验检测各组细胞增殖；划痕实验与Transwell实验检测各组细胞迁移与侵袭；Western blot实验检测各组细胞中P38/MAPK及p‑P38/MAPK的表达水平。 结果:S100A8在多种癌症高表达，S100A8高表达与膀胱癌患者不良预后相关；与人正常尿路上皮细胞株SV‑HUC‑1相比，膀胱癌细胞系HT‑1376中S100A8蛋白与mRNA均高表达；KD组中S100A8蛋白表达水平、24及48 h的OD₄₅₀值、新生成细胞的比例、迁移细胞数目、侵袭细胞数目、细胞中P38/MAPK及p‑P38/MAPK水平明显低于正常组及NC组（P<0.05）。OE组中S100A8蛋白表达水平、24及48 h的OD₄₅₀值、新生成细胞的比例、迁移细胞数目、侵袭细胞数目、细胞中P38/MAPK及p‑P38/MAPK水平明显高于正常组及NC组（P<0.05）。 结论:S100A8在膀胱癌细胞中高表达，S100A8通过调控P38/MAPK通路影响膀胱癌的增殖、侵袭和迁移。

Objective:To investigate the effect and mechanism of S100 calcium‑binding protein A8 （S100A8） on the proliferation， invasion and migration of bladder cancer. Methods:The expression of S100A8 in bladder cancer was investigated through TCGA database， HPA database and bladder cancer patients in the Department of Urology， the First Affiliated Hospital of Shihezi University. Bladder cancer cell line HT‑1376 with high expression of S100A8 and human normal urothelial cell line SV‑HUC‑1 were selected from CCLE database. Western blot and qRT‑PCR were used to verify the expression of S100A8. HT‑1376 cells were divided into the KD group， the NC group， the OE group， and the normal group， and the knockdown and overexpression lentivirus transfection were performed. Western blot and qRT‑PCR experiments were used to detect the transfection efficiency. CCK‑8 method and EdU proliferation test were used to detect cell proliferation in each group. Scratch test and Transwell test were used to detect cell migration and invasion. Western blot was used to detect the expression levels of P38/MAPK and p‑P38/MAPK in each group. Results:S100A8 was highly expressed in a variety of cancers. High expression of S100A8 was associated with poor prognosis of bladder cancer patients. S100A8 protein and mRNA were highly expressed in bladder cancer cell line HT‑1376 compared to human normal urothelial cell line SV‑HUC‑1. The expression level of S100A8 protein， OD₄₅₀ values at 24 and 48 h， the proportion of newly generated cells， the number of migrating cells， the number of invasive cells， and the levels of P38/MAPK and p‑P38/MAPK in cells in the KD group were significantly lower than those in the normal group and the NC group （P<0.05）. The expression level of S100A8 protein， OD₄₅₀ values at 24 and 48 h， the proportion of newly generated cells， the number of migrating cells， the number of invasive cells， and the levels of P38/MAPK and p‑P38/MAPK in the OE group were significantly higher than those in the normal group and the NC group （P<0.05）. Conclusion:S100A8 is highly expressed in bladder cancer cells， and S100A8 may affect the proliferation， invasion and migration of bladder cancer by regulating the P38/MAPK pathway.

R737.14

兵团科技计划项目（2024ZD057）；石河子大学科研项目（ZZZC202184）；石河子大学第一附属医院科研项目（BS202110）